Gideon Chukwuka Isika


This study investigated the concentrations of fractions of copper (Cu) and zinc (Zn) and their relationship with some soil physical and chemical properties. The sequential extraction procedure described by Oviasogie et al., (2011) was used to analyze composite soil samples collected at depths of 0-15cm, 15-30cm, 30-60cm, 60-90cm and 90-120cm from Idanre, NIFOR, Uhonmora, Dutse, Koko and Badagry. The fractions determined were the water soluble, exchangeable, carbonate bound, plant available, oxide bound, amorphous oxide bound, crystalline oxide bound, organic matter bound and residual fractions. The fractionation scheme revealed that the concentrations of water soluble, exchangeable, carbonate bound, plant available, and organic matter bound Cu and Zn fractions reduced with depth while oxide bound, amorphous oxide bound and crystalline oxide bound fractions had higher concentrations in the deeper horizons. Cu was primarily present in the bound to amorphous oxide, residual and plant available fractions, while Zn was predominant in the residual and crystalline oxide bound fractions. Zn occurred more than Cu in the entire soil profiles investigated. The reducible forms of copper and zinc showed negative correlation with pH, organic carbon, total N, available P, sand and silt while the water soluble, exchangeable, carbonate bound, plant available and organic matter bound fractions showed positive correlations with the aforementioned soil properties. Clay had positive correlations with the former and negative with the latter.


speciation, heavy metal, sequential extraction


Akcay, H., Oguz, A., and Karapire, C. (2003). Study of heavy metal pollution and speciation in Buyak Menders and Gedis river sediments. Water Resources. 37: 813-822.

Allison, L.E. (1965). Organic carbon. In: Methods of Soil Analysis, Part 2, C.A. Black et al., Ed. Agronomy 9:1367-1378. American Society of Agronomy. Inc., Madison, WI.

Ashish, B., Neeti, K., and Himanshu, K. (2013). Copper toxicity: A comprehensive study. Residual Journal of recent sciences. 2: 58-67

Bouyoucos, G.J. (1962). Hydrometer method improved for making particle size analysis of soils. Journal of Agronomy 54:464-465.

Bray, R. H., and Kurtz, L. T. (1945). Determination of total, organic, and available forms of phosphorus in soils. Soil Science. 59: 39 -45.

Bremner, J.M. (1965) “Total nitrogen. In: C.A. Black (Ed.), Methods of Soil Analysis, Part 2, American Society of Agronomy, Madison, pp. 1149-1178.

Ezeaguba, A. G. Okoye, P. A. C. Umeobika U. C. and Abugu H. O. (2016) Speciation Patterns of Some Selected Heavy Metals in the Soil along the Effluent Channels of Juhel Pharmeutical Industry, Awka, Anambra State, Nigeria. American Chemical Science Journal11(2): 1-14, 2016, Article no.ACSJ.21495

Kabala C. and Singh B. R. (2001). Fractionation and Mobility of Copper, Lead and Zinc in Soil Profiles in the vicinity of a copper smelter. J. Environ. Qual. 30: 485-492.

Kabata-Pendias, A., Pendias, H. (1992). ‘Trace elements in soils and plants’. London: CRC Press.

Maiz, I., Arambari, I., Garcia, R. and Millan, E. (2000). Evaluation of heavy metals availability in polluted soils by two sequential extraction processes using factor analysis. Environmental pollution. 110: 3-9.

Norvell, W. A., (1991). Reactions of metal chelates in soils and nutrient solutions. In “Micronutrients in Agriculture”, 2nd Ed. (ed. Mortvedt, J.J., Cox, F.R., Shuman, L. M. and Welch, R.M). Soil science society of America. Madison. Pp. 187-227

Oviasogie, P. O., Aghimien, A.E., and Ndiokwere, C. L (2011). Fractionation and bioaccumulation of copper and zinc in wetland soils of the Niger Delta determined by the oil palm. Chem. Speciation and Bioavailability 23(2). www.chemspecbio.co.uk

Papafilippaki, A.K., Kotti, M. E., and Stavroulakis, G. G (2008). Seasonal variation in dissolved heavy metals in the Keritis River, Chania, Greece. Global NEST Journal. 10(3): 320-325

Rautaray, S. K., Ghosh, B. C., Mittra, B. N., (2003). Effect of fly ask, organic wastes and chemical fertilizers on yield, nutrient uptake, heavy metal content, and residual fertility in a rice-mustard cropping sequence under acid lateritic soil. Bioresour. Technol. 90:275-283

Sposito, G., Lund, L. J., Chang, A. C., (1982). Trace metal chemistry in field soils amended with sewage sludge in the arid zone. In Fractionation of Ni, Cu, Zn, Cd and Pb in solid phases. Soil Sci. Soc. Am. J. 46: 260-264

Tessier A., Campbell P.G.C., and Bisson M. (1979) Sequential extraction procedure for the speciation of Particulate trace metals. Analytical Chemistry. 51: 844-851.


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